The Biophysics Division at the Department of Biology of the Osnabrück University promotes research and teaching in molecular and cellular biophysics. Our mission is to bridge the gap between the biological and the physicochemical perspective on cellular processes. To this end we have developed a broad spectrum of biophysical tools for quantifying molecular functions in complex environment, often in the context of lipid membranes. We apply these tools for unraveling the mechanistic determinants of signal transduction across the plasma membrane and we share them in numerous collaborations at the Department of Biology and beyond. Within several Bachelor and Master Courses, we introduce the inherently interdisciplinary concepts of biophysics, which we believe are pivotal for fundamental progress in the understanding of life.